Pulsed-field gel electrophoresis for epidemiologic studies of Campylobacter hyointestinalis isolates
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چکیده
منابع مشابه
Campylobacter Epidemiology – Insights from Subtyping by Pulsed-Field Gel Electrophoresis
Höök, H. 2005. Campylobacter epidemiology – insights from subtyping by pulsed-field gel electrophoresis. Doctoral thesis. ISSN 1652-6880, ISBN 91-576-6988-0 Campylobacter jejuni and Campylobacter coli are frequent worldwide causes of food-borne gastroenteritis. Poultry is regarded as the most important infection source. In temperate countries, a marked seasonal variation of human campylobacteri...
متن کاملPulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
متن کاملPulsed-field Gel Electrophoresis for Salmonella Infection
To detect Salmonella clusters, public health laboratories perform pulsed-field gel electrophoresis (PFGE) that provides a PFGE pattern, or DNA fingerprint. If the PFGE patterns of isolates from >2 persons are indistinguishable, the responsible bacteria may be related to a common source (1–3). PulseNet is a network of public health laboratories coordinated by the Centers for Disease Control and ...
متن کاملPulsed-field gel electrophoresis of circular DNA.
Mobility of supercoiled (form I) and nicked circular (form II) plasmid DNAs was determined on two major forms of pulsed-field electrophoresis, CHEF and OFAGE. Plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with Saccharomyces cerevisiae chromosomes as standards. Agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels re...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1992
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.30.8.1982-1984.1992